| Solution Information | help | |
| Enzyme: | Orexin/Hypocretin receptor type 1 | |
| inhibitor: | BDBM79957 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine dose response curves for compounds identified as active in a set of previous experiments entitled, "Fluorescence-based cell-based primary high throughput screening assay to identify antagonists of the orexin 1 receptor (OX1R; HCRTR1) (AID 434989), and that confirmed activity in a previous set of experiments entitled, "Fluorescence-based cell-based high throughput confirmation assay for antagonists of the orexin 1 receptor (OX1R; HCRTR1)" (AID 492963). Compounds tested in this assay were inactive in a set of experiments entitled, "Fluorescence-based counterscreen for orexin 1 receptor (OX1R) antagonists: cell-based high throughput assay for antagonists of the parental CHO cell line, in triplicate" (AID 492965). In this assay CHO cells stably transfected with the human OX1R receptor (CHO/OX1R cell line) are treated with test compounds, followed by measurement of intracellular calcium as monitored by the Fluo-8 fluorescent, cell | |
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